Figure 3.

RNA integrity checking. Gel image generated by the automated electrophoresis system (Experion, Biorad). Lanes M – RNA molecular weight markers supplied with the Experion RNA StdSens analysis kit. Lanes PGTX beads – 1 μl of extracted RNA from distinct extractions using the PGTX solution combined with mechanical cell disruption with glass beads. Lanes Trizol beads – 1 μl of extracted RNA from distinct extractions using Trizol combined with mechanical cell disruption with glass beads. Lanes PGTX 95 – 1 μl of extracted RNA from distinct extractions using the PGTX solution combined with high temperature cell disruption. Lanes Trizol 95 – 1 μl of extracted RNA from distinct extractions using Trizol combined with high temperature cell disruption. Lanes Trizol std – 1 μl of extracted RNA from distinct extractions using Trizol according to general manufacturer's instructions. Lanes BPC – 1 μl of extracted RNA from distinct extractions using the BPC method. RNA integrity was kept for all extraction methods.

Pinto et al. BMC Molecular Biology 2009 10:79   doi:10.1186/1471-2199-10-79
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