Table 3

Identification of proteins in Western blots

ruPAR

HASM

HBEC

BEAS2B

THP1

PMN

PBMC

Predicted variants


70

70

dimer/aggregates

50–60

60

60

55–60

60

60

60

full length

anti-D1

(55-50)

50

(50)

alternative ex7b

(IIIF10) (kDa)

45

(45)

single exon deletion (e.g. exon 3 or 6)

(37)

(37)

37

37

37

two exon deletions (e.g. exon 5+6)

30

(30)

alternative ex7b, exon 4+5deletion


75

(75)

(75)

75

75

dimer/aggregates

anti-D2

50–55

60

(60)

60

60

50

full length

(3932)

(kDa)

45

45

45

45

45

45

single exon deletion or alternative ex7b

(40)

40

40

two exon deletions

(37)

37

(37)

37

37

37

alternative ex7b, exon 4+5deletion or D2/3 fragment


Western blots were performed using two antibodies specific to domains 1 and 2 of the uPAR protein respectively (Figure 9). The main proteins observed in each cell or tissue type with each antibody are summarised. Approximate molecular weights of bands are shown (kDa), with weaker bands shown in brackets and the strongest band in bold. Potential splice variants for each molecular weight are also shown.

Stewart and Sayers BMC Molecular Biology 2009 10:75   doi:10.1186/1471-2199-10-75

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