The effect of using different concentrations of FGEL in blocking the beads. Relative enrichment was calculated for EKLF sequences over chromatin from non transfected control cells. Low enrichment of the EKLF promoter and enhancer sequences is observed when blocking with salmon sperm DNA alone (minus FGEL control) due to the high background binding obtained with chromatin from the non-transfected control cells.
Kolodziej et al. BMC Molecular Biology 2009 10:6 doi:10.1186/1471-2199-10-6