Preclearing chromatin with Protein G Dynabeads. A) Relative enrichment of EKLF sequences calculated over chromatin from control cells. The enrichment of the specific EKLF promoter elements appears lower after preclearing presumably due to some loss of chromatin in the additional preclearing step. B) Relative enrichment of biotin-tagged GATA-1 binding at EKLF promoter and enhancer calculated over the negative control sequence for biotin-tagged GATA-1 chromatin and chromatin from BirA expressing cells as negative control.
Kolodziej et al. BMC Molecular Biology 2009 10:6 doi:10.1186/1471-2199-10-6