ColR binding sites in the promoter regions of oprQ and PP0903. (a) DNase I footprinting assay performed on oprQ and PP0903 promoter DNA. Dideoxy-chain termination sequences corresponding to the probes are shown in the order of G, A, T and C. Concentrations of DNA-binding protein ColR, its cognate kinase ColS, and phosphoryl-donor ATP are shown above each lane. Vertical black lines indicate ColR-protected regions and dot represents a site hypersensitive to DNase I cleavage. (b) Sequences of ColR-protected regions in the promoters of oprQ and PP0903. A site hypersensitive to DNase I cleavage in the presence of ColR is in bold. Nucleotides identical in two ColR binding sites are underlined. Distance of the ColR-binding site from the start codon is presented.
Kivistik et al. BMC Molecular Biology 2009 10:46 doi:10.1186/1471-2199-10-46