ColR activates the expression of its downstream-locating PP0903. (a) Organization of genes in P. putida colRS locus. (b) β-galactosidase (β-gal) activities measured in P. putida wild-type strain PaW85 (wt), colR-defective strain PaWcolR (colR) and colR-defective strain PaWRtaccolR complemented with colR gene under the control of the inducible Ptac promoter (RtacR). All strains carried the reporter plasmid with the promoter of PP0903. ColR expression in strain PaWRtaccolR was induced with 0.5 mM IPTG (RtacR+IPTG). Bacteria were grown either on glucose (glc) or glucose plus 2.5 mM phenol (glc+phe) minimal plates. Data (means and standard deviations) from at least three independent experiments are presented. For promoter probe vector p9TTBlacZ, the basal level of β-galactosidase activity was less than 0.5 Miller units.
Kivistik et al. BMC Molecular Biology 2009 10:46 doi:10.1186/1471-2199-10-46