Map of the major adenovirus type 5 E1A proteins and transcriptional activation by LexA-E1A fusions. A) The two major products of E1A are 289 and 243 residues (R) in length and differ only by the presence of an additional 46 amino acids unique to the larger protein. Regions of sequence conservation relevant to this study (CR) are shown as are the regions expressed as LexA DBD fusions. B) Yeast strain BY4741 was transformed with the pSH1834 LexA responsive reporter plasmid and vectors expressing the LexA DBD, or the LexA DBD fused to the indicated portions of E1A. Extracts were prepared from transformed yeast and assayed for β-galactosidase activity as described previously . Experiments were performed in triplicate with s.d's indicated.
Yousef et al. BMC Molecular Biology 2009 10:32 doi:10.1186/1471-2199-10-32