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Open Access Research article

Requirements for E1A dependent transcription in the yeast Saccharomyces cerevisiae

Ahmed F Yousef1, Christopher J Brandl2 and Joe S Mymryk13*

Author Affiliations

1 Department of Microbiology & Immunology, University of Western Ontario, London, Ontario, Canada

2 Department of Biochemistry, University of Western Ontario, London, Ontario, Canada

3 Department of Oncology, University of Western Ontario, London, Ontario, Canada

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BMC Molecular Biology 2009, 10:32  doi:10.1186/1471-2199-10-32

Published: 17 April 2009

Additional files

Additional file 1:

Western Blot analysis of LexA-DBD-CR3 expression levels in selected yeast disruption strains. The data provided show the relative levels of expression of LexA-DBD-CR3 in selected yeast strains.

Format: EPS Size: 455KB Download file

Open Data

Additional file 2:

Fold activation relative to wild-type in different yeast deletion strains isogenic to BY4741. The data provided show the relative fold activation of LexA-DBD-N-terminus and LexA-DBD-CR3 in additional yeast disruption strains.

Format: XLS Size: 24KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Reporter copy number variation in yeast disruption strains and effect of different methods of preparing yeast extract on E1A-dependent transcriptional activation. The data provided show the effect of changing the copy number of the reporter or method of preparing yeast extracts on E1A dependent transcriptional activation in selected yeast strains.

Format: EPS Size: 138KB Download file

Open Data

Additional file 4:

List of all yeast strains and their sources that were used in this study. This table lists all yeast strains and their sources that were used in this study.

Format: XLS Size: 35KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 5:

Raw data representing average "Miller units" obtained from performing the experiments described in the Methods. This table lists all the raw data obtained in this study.

Format: XLS Size: 48KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data