Email updates

Keep up to date with the latest news and content from BMC Molecular Biology and BioMed Central.

Open Access Research article

Requirements for E1A dependent transcription in the yeast Saccharomyces cerevisiae

Ahmed F Yousef1, Christopher J Brandl2 and Joe S Mymryk13*

Author Affiliations

1 Department of Microbiology & Immunology, University of Western Ontario, London, Ontario, Canada

2 Department of Biochemistry, University of Western Ontario, London, Ontario, Canada

3 Department of Oncology, University of Western Ontario, London, Ontario, Canada

For all author emails, please log on.

BMC Molecular Biology 2009, 10:32  doi:10.1186/1471-2199-10-32

Published: 17 April 2009

Abstract

Background

The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two regions that can independently induce transcription when fused to a heterologous DNA binding domain. When expressed in Saccharomyces cerevisiae, each of these regions of E1A also acts as a strong transcriptional activator. This allows yeast to be used as a model system to study mechanisms by which E1A stimulates transcription.

Results

Using 81 mutant yeast strains, we have evaluated the effect of deleting components of the ADA, COMPASS, CSR, INO80, ISW1, NuA3, NuA4, Mediator, PAF, RSC, SAGA, SAS, SLIK, SWI/SNF and SWR1 transcriptional regulatory complexes on E1A dependent transcription. In addition, we examined the role of histone H2B ubiquitylation by Rad6/Bre1 on transcriptional activation.

Conclusion

Our analysis indicates that the two activation domains of E1A function via distinct mechanisms, identify new factors regulating E1A dependent transcription and suggest that yeast can serve as a valid model system for at least some aspects of E1A function.