Figure 4.

The putative translation products from alternative splicing of Mus musculus Rad51d fail to complement a Rad51d deletion. (A) Alternative transcripts of Rad51d were expressed in Rad51d^-/-Trp53^-/- MEFs. The percentage resistance indicates the fraction of transfectants that survived treatment with 4 ng/mL mitomycin C (MMC). Cells transfected with empty vector (vec) were included as controls. Error bars represent the standard error. (B) Western blot of HA-tagged proteins expressed in Rad51d^-/-Trp53^-/- MEFs 24 hr post-transfection (upper panel). The expected molecular mass of each isoform is shown above each. Immunoblotting for β-tubulin was performed as a loading control (lower panel). Expression of RAD51DΔ3 could not be verified likely due to the relatively small molecular weight of the putative translation product (6.7 kDa).