The putative translation products from alternative splicing of Mus musculus Rad51d fail to complement a Rad51d deletion. (A) Alternative transcripts of Rad51d were expressed in Rad51d-/-Trp53-/- MEFs. The percentage resistance indicates the fraction of transfectants that survived treatment with 4 ng/mL mitomycin C (MMC). Cells transfected with empty vector (vec) were included as controls. Error bars represent the standard error. (B) Western blot of HA-tagged proteins expressed in Rad51d-/-Trp53-/- MEFs 24 hr post-transfection (upper panel). The expected molecular mass of each isoform is shown above each. Immunoblotting for β-tubulin was performed as a loading control (lower panel). Expression of RAD51DΔ3 could not be verified likely due to the relatively small molecular weight of the putative translation product (6.7 kDa).
Gruver et al. BMC Molecular Biology 2009 10:27 doi:10.1186/1471-2199-10-27