Research article
Evidence of a tick RNAi pathway by comparative genomics and reverse genetics screen of targets with known loss-of-function phenotypes in Drosophila
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* Corresponding author: Ala E Lew-Tabor ala.lew@dpi.qld.gov.au
- † Equal contributors
1 Cooperative Research Centre for Beef Genetic Technologies, Armidale, NSW, Australia
2 Centre for Comparative Genomics (CCG), Murdoch University, Perth, Western Australia 6150, Australia
3 Department of Primary Industries and Fisheries, Emerging Technologies, Locked Mail Bag No. 4, Moorooka 4105, Queensland, Australia
4 Department of Entomology, University of Minnesota, St Paul, Minnesota 55108, USA
5 USDA-ARS, Knipling Bushland US Livestock Insect Research Laboratory, 2700 Fredericksburg Road, Kerrville, TX 78028, USA
BMC Molecular Biology 2009, 10:26 doi:10.1186/1471-2199-10-26
Published: 26 March 2009Additional files
Additional File 1:
Table of conserved domains of Dicer proteins identified in R. microplus ESTs with details on ORF length, domain positions, result scores of Pfam search, and scores of BLAST searches. List of Dicer domains identified ESTs in R. microplus.
Format: XLS Size: 48KB Download file
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Additional File 2:
Bioinformatics analysis pipeline. A dataflow diagram of the bioinformatics analysis pipeline used in the identification of RNAi targets.
Format: PPT Size: 57KB Download file
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Additional File 3:
Gene Ontology terms distribution of R. microplus sequences. A schematic representation of the functional relationship of the R. microplus genes targeted in the RNAi cell culture and in vivo experiments, based on Gene Ontology terms assigned by InterProScan searches.
Format: PPT Size: 79KB Download file
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Additional File 4:
Sequences used in the identification of conserved regions for the design of primers for the PCR amplification of R. microplus homologues of D. melanogaster known RNAi phenotypes. List of GenBank accessions used to identify conserved regions to assist with primer design for the amplification of dsRNA treatments.
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Additional File 5:
Sequences of oligonucleotides used for the amplification of template DNA for subsequent in vitro transcription of dsRNA.
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Additional File 6:
GenBank accessions for clones for R. microplus tentative consensus sequences identified in this study.
Format: XLS Size: 38KB Download file
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