Table 5 |
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|
Ranking of potential reference genes according to the expression stability |
||
|
Tissues |
Analsysis |
Ranking |
|
|
||
|
All endocrine tissues tested |
NF |
YWHAZ > GUSB > B2M > RPS7> HMBS > ABL > 18S > GAPDH > ACTB > HPRT |
|
gN(M) |
B2M > GUSB > YWHAZ > HMBS > RPS7 > ABL > 18S > GAPDH > ACTB > HPRT |
|
|
gN |
B2M = HMBS > GUSB > YWHAZ > RPS7 > ABL > 18S > GAPDH > ACTB > HPRT |
|
|
Adrenal gland |
NF |
HPRT > RPS7 > ABL > B2M > ACTB > GAPDH > 18S > HMBS > YWHAZ > GUSB |
|
gN(M) |
HPRT > RPS7 > ABL > ACTB > B2M > 18S = GAPDH > HMBS > YWHAZ > GUSB |
|
|
gN |
GAPDH = 18S > RPS7 > HPRT > YWHAZ > ABL > B2M > ACTB > HMBS > GUSB |
|
|
Pancreas |
NF |
RPS7 > GUSB > YWHAZ > HMBS > B2M > GAPDH > ABL > 18S > HPRT > ACTB |
|
gN(M) |
RPS7 > GUSB > YWHAZ > B2M > HMBS > ABL > GAPDH > 18S > HPRT > ACTB |
|
|
gN |
GUSB = RPS7 > YWHAZ > HMBS > B2M > ABL > 18S > GAPDH > HPRT > ACTB |
|
|
Parathyroid |
NF |
ACTB > RPS7 > GAPDH > HMBS > YWHAZ > ABL > B2M > GUSB > 18S > HPRT |
|
gN(M) |
ACTB > RPS7 > GAPDH > ABL > HMBS > YWHAZ > B2M > GUSB > 18S > HPRT |
|
|
gN |
GAPDH = RPS7 > ACTB > HMBS > ABL > YWHAZ > B2M > GUSB > 18S > HPRT |
|
|
Thyroid |
NF |
GUSB > ACTB > YWHAZ > HMBS > B2M > RPS7 > ABL > 18S > GAPDH > HPRT |
|
gN(M) |
ACTB > GUSB > YWHAZ > B2M > HMBS > RPS7 > ABL > 18S > GAPDH > HPRT |
|
|
gN |
ACTB = HMBS > GUSB > B2M > YWHAZ > RPS7 > ABL > 18S > GAPDH > HPRT |
|
|
All lymphoid tissues tested |
NF |
RPS7 > GUSB > ACTB > YWHAZ > ABL > GAPDH > B2M > HPRT > 18S > HMBS |
|
gN(M) |
GUSB > RPS7 > ACTB > YWHAZ > ABL > B2M > GAPDH > HPRT > 18S > HMBS |
|
|
gN |
GUSB = RPS7 > ACTB > YWHAZ > ABL > B2M > GAPDH > HPRT > 18S > HMBS |
|
|
Bone marrow |
NF |
RPS7 > HMBS > ABL > GUSB > YWHAZ > ACTB > B2M > 18S > HPRT > GAPDH |
|
gN(M) |
RPS7 > HMBS > ABL > GUSB > YWHAZ > ACTB > B2M > 18S > HPRT > GAPDH |
|
|
gN |
ACTB = YWHAZ > HMBS > GUSB > RPS7 > ABL > B2M > 18S > HPRT > GAPDH |
|
|
Lymph node |
NF |
GUSB > ACTB > B2M > HMBS > YWHAZ > ABL > RPS7 > GAPDH > 18S > HPRT |
|
gN(M) |
GUSB > B2M > ACTB > YWHAZ > ABL > HMBS > RPS7 > GAPDH > 18S > HPRT |
|
|
gN |
ABL = B2M > YWHAZ > ACTB > GUSB > HMBS > GAPDH > RPS7 > 18S > HPRT |
|
|
Spleen |
NF |
GUSB > RPS7 > ACTB > ABL > B2M > HMBS > YWHAZ > HPRT > GAPDH > 18S |
|
gN(M) |
GUSB > RPS7 > ACTB > B2M > ABL > HMBS > YWHAZ > HPRT > GAPDH > 18S |
|
|
gN |
ACTB = B2M > GUSB > RPS7 > ABL > HMBS > YWHAZ > HPRT > GAPDH > 18S |
|
|
All gastrointestinal tissues tested |
NF |
ACTB > HMBS > YWHAZ > RPS7 > ABL > GAPDH > GUSB > B2M > 18S > HPRT |
|
gN(M) |
ACTB > HMBS > YWHAZ > RPS7 > ABL > GAPDH > GUSB > B2M > 18S > HPRT |
|
|
gN |
ABL = HMBS > RPS7 > ACTB > YWHAZ > GAPDH > B2M > GUSB > 18S > HPRT |
|
|
Parotid gland |
NF |
ABL > ACTB > RPS7 > YWHAZ > HMBS > B2M > HPRT > GAPDH > GUSB > 18S |
|
gN(M) |
ABL > RPS7 > ACTB > YWHAZ > HMBS > B2M > HPRT > GAPDH > GUSB > 18S |
|
|
gN |
ABL = ACTB > HMBS > RPS7 > YWHAZ > B2M > HPRT > GAPDH > GUSB > 18S |
|
|
Duodenum |
NF |
RPS7 > GAPDH > ACTB > HMBS > YWHAZ > ABL > 18S > GUSB > B2M > HPRT |
|
gN(M) |
RPS7 > GAPDH > ACTB > HMBS > YWHAZ > ABL > 18S > GUSB > B2M > HPRT |
|
|
gN |
ACTB = HMBS > RPS7 > GAPDH > YWHAZ > ABL > 18S > GUSB > B2M > HPRT |
|
|
Ileum |
NF |
HMBS > ABL > YWHAZ > ACTB > GAPDH > GUSB > RPS7 > HPRT > B2M > 18S |
|
gN(M) |
HMBS > ABL > ACTB > YWHAZ > GAPDH > GUSB > RPS7 > HPRT > B2M > 18S |
|
|
gN |
YWHAZ = ACTB > HMBS > ABL > GUSB > GAPDH > RPS7 > HPRT > B2M > 18S |
|
|
Liver |
NF |
GUSB > GAPDH > RPS7 > HPRT > HMBS > YWHAZ > ABL > ACTB > B2M > 18S |
|
gN(M) |
GAPDH > HMBS > HPRT > GUSB > RPS7 > YWHAZ > ABL > ACTB > B2M > 18S |
|
|
gN |
ACTB = HMBS > ABL > GAPDH > HPRT > GUSB > YWHAZ > RPS7 > B2M > 18S |
|
|
Kidney |
NF |
YWHAZ > RPS7 > ABL > ACTB > HMBS > GUSB > GAPDH > B2M > HPRT > 18S |
|
gN(M) |
YWHAZ > RPS7 > ABL > ACTB > HMBS > GUSB > GAPDH > B2M > HPRT > 18S |
|
|
gN |
RPS7 = YWHAZ > ABL > ACTB > HMBS > GAPDH > GUSB > B2M > HPRT > 18S |
|
|
Myocardium |
NF |
ACTB > RPS7 > GAPDH > HMBS > YWHAZ > ABL > GUSB > 18S > HPRT > B2M |
|
gN(M) |
RPS7 > ACTB > GAPDH > YWHAZ > HMBS > ABL > GUSB > 18S > HPRT > B2M |
|
|
gN |
GAPDH = YWHAZ > RPS7 > ACTB > HMBS > ABL > GUSB > 18S > HPRT > B2M |
|
|
Brain |
NF |
ACTB > ABL > RPS7 = YWHAZ > HMBS > GAPDH > HPRT > GUSB > B2M > 18S |
|
gN(M) |
ACTB > ABL > RPS7 > YWHAZ > HPRT > GAPDH > HMBS > GUSB > B2M > 18S |
|
|
gN |
ABL = RPS7 > ACTB > GAPDH > HPRT > YWHAZ > HMBS > GUSB > B2M > 18S |
|
|
All healthy tissues combined |
NF |
RPS7 > GUSB > YWHAZ > ABL > ACTB > B2M > HMBS > 18S > GAPDH > HPRT |
|
gN(M) |
RPS7 > GUSB > YWHAZ > ABL > ACTB > B2M > HMBS > 18S > GAPDH > HPRT |
|
|
gN |
GUSB = RPS7 > ABL > YWHAZ > ACTB > B2M > HMBS > 18S > GAPDH > HPRT |
|
|
Blood |
NF |
YWHAZ > ABL > GAPDH > B2M > ACTB > GUSB > 18S > HPRT > RPS7 > HMBS |
|
gN(M) |
YWHAZ > B2M > GAPDH > ABL > ACTB > 18S > GUSB > HPRT > RPS7 > HMBS |
|
|
gN |
YWHAZ = B2M > ACTB > 18S > GAPDH > ABL > GUSB > HPRT > RPS7 > HMBS |
|
|
Neoplastic tissues |
NF |
RPS7 > ACTB > HMBS > GAPDH > B2M > ABL > 18S > GUSB > YWHAZ > HPRT |
|
gN(M) |
ACTB > RPS7 > HMBS > GAPDH > B2M > 18S > ABL > GUSB > YWHAZ > HPRT |
|
|
gN |
GAPDH = HMBS > 18S > RPS7 > ACTB > B2M > ABL > GUSB > YWHAZ > HPRT |
|
|
|
||
|
The expression stability is given as stability values calculated by NormFinder (NF), M values calculated by geNorm (gN(M)), and geNorm calculations (gN). Rankings are shown from most (left) to least (right) stable genes. |
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|
Kessler et al. BMC Molecular Biology 2009 10:106 doi:10.1186/1471-2199-10-106 |
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