Table 1

Descriptive statistics and expression level analyses of the tested candidate reference genes based on their crossing point (CP) values

Factor

actin

adh

14-3-3

ccs

gapdh

poly

rpl7

cys


N

21

21

21

21

21

21

21

21

GM [CP]

32.72

29.7

29.63

26.67

22.86

28.46

30.76

26.67

AM [CP]

32.74

29.71

29.64

26.67

22.88

28.49

30.77

26.7

Min [CP]

31.4

28.64

28.94

25.92

21.74

26.79

30.13

25.24

Max [CP]

34.35

31.12

31.21

27.37

23.77

30.07

31.93

28.44

SD [± CP]

1.23

0.99

0.92

0.58

0.94

1.39

0.69

1.31

CV [%CP]

3.75

3.35

3.09

2.19

4.12

4.89

2.24

4.89

Min [x-fold]

0.41

0.43

0.66

0.53

0.54

0.18

0.68

0.41

Max [x-fold]

3.04

3.1

2.62

1.83

1.64

5.22

2.02

3.04

SD [± x-fold]

1.07

1.08

0.82

0.52

0.52

2.09

0.53

1.08


Abbreviations: N: number of samples; CP: crossing-point; GM [CP]: geometric CP mean; AM [CP]: arithmetic CP mean; Min [CP] and Max [CP]: CP threshold values; SD [± CP]: CP standard deviation; CV [%CP]: variance coefficient expressed as percentage of CP level; Min [x-fold] and Max [x-fold]: threshold expression levels expressed as absolute x-fold over- or under-regulation coefficient; SD [± x-fold]: standard deviation of absolute regulation coefficient.

Barsalobres-Cavallari et al. BMC Molecular Biology 2009 10:1   doi:10.1186/1471-2199-10-1

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