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Open Access Highly Accessed Open Badges Research article

Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions

Carla F Barsalobres-Cavallari1*, Fábio E Severino1, Mirian P Maluf2 and Ivan G Maia1

Author Affiliations

1 Laboratório de Biotecnologia e Genética Molecular, Departamento de Genética, Instituto de Biociências, UNESP, Distrito de Rubião Júnior s/n, 18618-000, Botucatu, São Paulo, Brazil

2 Embrapa/IAC, Centro de Café Alcides Carvalho, Campinas, São Paulo, Brazil

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BMC Molecular Biology 2009, 10:1  doi:10.1186/1471-2199-10-1

Published: 6 January 2009

Additional files

Additional file 1:

Results from Bestkeeper descriptive statistical analysis. The data provided represent the descriptive statistics, based on crossing point (CP) values, for the expression analyses of the candidate reference genes in the five distinct coffee plant tissue/organ set.

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Additional file 2:

Coffee tissue/organ sample set (Coffea arabica var. Mundo Novo – IAC 388-17-1) used in the present study. Freshly harvested roots, stems, and leaves were obtained from 4 month-old coffee plants grown under greenhouse conditions (28°C, 60% RH) in Campinas, São Paulo, Brazil. Flower and fruit samples, at different developmental stages, were collected from 4–5 year-old field grown plants in Botucatu and in Campinas, São Paulo, Brazil. After harvesting, fresh tissue samples were frozen immediately in liquid nitrogen until RNA extraction.

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