Figure 2.

Full length HOXD13 cDNA PCR product analysis. 1) 1.1 kb full length homeobox D13 cDNA generated by using DF1 and DR2 specific primers. 2 and 3) 100 bp ladder (Gibco BRL Life Technologies, Rockville, MD) 4). Restriction endonuclease digestion of PCR product with Ppum1 showing correct expected size fragments of 519, 316 and 265 bps. respectively 5). 1 kb ladder (Gibco BRL Life Technologies, Rockville, MD) 6-8). are restriction digestion fragments of full length HOXD13 cloned in PCR II (vector) with EcoR1 (1.1 kb), Kpn 1 (381 bp) and Sma1 and BamH1 (826 bp) respectively.

Wang et al. BMC Molecular Biology 2000 1:2   doi:10.1186/1471-2199-1-2
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