Open Access Highly Accessed Research article

Genomic analysis of Campylobacter fetus subspecies: identification of candidate virulence determinants and diagnostic assay targets

Paula M Moolhuijzen1*, Ala E Lew-Tabor12, Bartosz M Wlodek2, Fernán G Agüero3, Diego J Comerci3, Rodolfo A Ugalde3, Daniel O Sanchez3, Rudi Appels1 and Matthew Bellgard1

Author Affiliations

1 Centre for Comparative Genomics, School for Information Technology, Murdoch University, Murdoch, Western Australia, Australia

2 Emerging Technologies, Queensland Department of Primary Industries & Fisheries, Yeeongpilly, Queensland, Australia

3 Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín – CONICET, 1650 KNA, San Martín, Buenos Aires, Argentina

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BMC Microbiology 2009, 9:86  doi:10.1186/1471-2180-9-86

Published: 8 May 2009

Additional files

Additional File 1:

List of C. fetus subsp. venerealis specific ORF and ORF protein analyses record. The data provided represent the Blast analysis of C. fetus subsp. venerealis specific ORF against protein dataset. Table lists contig ORF, ORF contig position, protein accession, protein description, expected value of orf alignment to the protein sequence and percentage identities in the alignment.

Format: XLS Size: 89KB Download file

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Additional File 2:

List of C. fetus virulence gene contigs targeted in PCR assays. The data provided represent the Blast analysis of C. fetus subsp. venerealis specific ORF against protein dataset. Table lists contig ORF, ORF contig position, protein accession, protein description, expected value of orf alignment to the protein sequence and percentage identities in the alignment.

Format: XLS Size: 30KB Download file

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Additional File 3:

Supplemental Tables. Table S1, Table S2 and Table S3.

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Additional File 4:

Campylobacter proteome matrix analysis. An alignment Matrix displays protein similarity between the available Campylobacter complete proteomes (protein) and Cfv ORF (translated to amino acid). Percentage gene duplication is displayed as a percentage and as a heat map within species and across species and stains.

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Additional File 5:

Plasmid pCFV108 protein alignment to Campylobacter fetus venerealis ORFs. Diagram shows Plasmid pCFV108 and AZUL-94 Contig1185 ORF homology, Campylobacter homology is shaded in pink. Contig1185.orf00004 aligns to MobA (ABK41363) and Contig1185.orf00007 aligns to RepE (ABK41364).

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Additional File 6:

Campylobacter fetus venerealis genome sequencing and assembly data. Campylobacter fetus venerealis genome sequencing and assembly information.

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