Additional file 1.

PCR-RFLP of the ccrB gene using endonucleases HinfI and HinfI/BsmI. The figure provided shows the profiles of SCCmec types III and IV using the method of Yang et al. [40]. In lanes 1 and 3 ccrB amplicons are cut with HinfI whereas in lanes 2 and 4 the amplicons are cut with HinfI and BsmI. Lanes 1 and 2: S. epidermidis DF2LAB, SCCmec type III (537, 106 bp and 320, 174, 106 bp respectively); lanes 3 and 4: S. epidermidis V1LD1, SCCmec type IV (264, 227, 154 and 227, 171, 153, 93 bp respectively); M, molecular weight marker.

Format: PDF Size: 46KB Download file

This file can be viewed with: Adobe Acrobat Reader

Delgado et al. BMC Microbiology 2009 9:82   doi:10.1186/1471-2180-9-82