Research article
Identification of Archaea-specific chemotaxis proteins which interact with the flagellar apparatus
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* Corresponding authors: Matthias Schlesner schlesne@biochem.mpg.de - Dieter Oesterhelt oesterhe@biochem.mpg.de
1 Department of Membrane Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
2 Molecular Network Analysis Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany
3 Institute of Microbiology, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany
BMC Microbiology 2009, 9:56 doi:10.1186/1471-2180-9-56
Published: 16 March 2009Additional files
Additional File 1:
Protein-protein interaction analysis. This file provides additional information about the protein-protein interaction analysis. There are a figure and a table (Figure S1 and Table S1) detailing the results presented in Figure 2. Additionally, a figure illustrating the applied methods (Figure S2) and a detailed description of the methods are included.
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Additional File 2:
Confirmation of deletion strains by Southern blot analysis. Each deletion strain was probed with DIG-labeled 500 bp upstream sequence of the target gene(s) (us probe) and DIG-labeled target sequence (gene probe). Deletion strains are labeled according to their host strain (R1 or S9) followed by a Δ and the last digit of the identifier(s) of the deleted gene(s). 1 and 2 indicate the clones of the respective deletion that showed the expected bands and were used for further analysis, wt indicates the corresponding wild type. The upstream probe for OE2401F revealed an additional band, probably due to unspecific binding. This band, however, did not affect the significance of the blot.
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Additional File 3:
Swarming ability of the deletion strains. Swarm plates for the deletion strains in R1 and S9 background are shown. On each plate, the deletion strain (bottom) is compared to the respective wildtype strain (top). For each deletion in both host strains, two clones were tested (C1 and C2). Each clone was examined on two plates.
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Additional File 4:
Results of computer-based cell-tracking experiments. This table contains the detailed results from the computer-based cell-tracking experiments.
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Additional File 5:
Phenotype of complementations. A Swarm plate assay. On each plate the complementation strain (bottom) is compared to the respective wildtype strain (top). B Computer-based cell tracking for the complementations of each single deletion. The percent reversal in a 4 second interval was determined either without stimulation (spontaneous, gray bar) or after a blue light pulse (blue bar). Error bars represent the 95% confidence interval.
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Additional File 6:
Occurrence of che and fla genes in archaeal genomes. An exhaustive search for che and fla genes in archaeal genomes is presented and the detected orthologs listed as table (Table S2). Additionally, the method used for ortholog identification is described.
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Additional File 7:
Primers used in this study. This table lists the oligonucleotides used in the present study.
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