Table 2 |
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|
Microarray and real time PCR analysis showing a limited list of genesa predicted to be PreAB activated |
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|
ORF |
Gene |
Function |
Microarray Ab Md (fold change) |
Microarray Bc M (fold change) |
qRT-PCRe |
|
|
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|
STM3707 |
yibD |
putative glycosyltransferase |
0.8 (1.7) |
6.1 (68.6) |
NP f |
|
STM3176 |
ygiW |
Membrane protein (DUF388; exporter?) |
4.5 (22.6) |
5.2 (36.8) |
355 |
|
STM1253 |
Cytochrome b561 (Ni2+ dependent) |
2.9 (7.5) |
4.9 (29.9) |
372 |
|
|
STM1595 |
srfC |
ssrAB activated gene; predicted coiled-coil structure |
4.3 (19.7) |
4.7 (26.0) |
1.2 |
|
STM3175 |
putative bacterial regulatory helix-turn-helix proteins, AraC family |
3.6 (12.1) |
4.4 (21.1) |
605.3 |
|
|
STM1685 |
ycjX |
putative ATPase |
2.3 (4.9) |
3.8 (13.9) |
37.7 |
|
STM1252 |
putative cytoplasmic protein |
1.5 (2.8) |
2.8 (7.0) |
8.6 |
|
|
STM3179 |
mdaB |
NADPH specific quinone oxidoreductase (drug modulator) |
1.0 (2.0) |
2.8 (7.0) |
32.5 |
|
STM1684 |
ycjF |
putative inner membrane protein |
1.1 (2.1) |
2.6 (6.1) |
61.2 |
|
STM4291 |
pmrB |
sensory kinase in two-component regulatory system with PmrA |
ND g |
2.1 (4.3) |
NP |
|
STM2080 |
udg |
UDP-glucose/GDP-mannose dehydrogenase |
ND |
1.8 (3.5) |
23.2 |
|
STM4292 |
pmrA |
response regulator in two-component regulatory system with PmrB |
ND |
1.7 (3.2) |
NP |
|
STM4118 |
yijP (cptA) |
putative integral membrane protein |
ND |
1.5 (2.8) |
32.8 |
|
STM0628 |
pagP |
PhoP-activated gene, palmitoyl transferase |
ND |
1.1 (2.1) |
NP |
|
STM2238 |
putative phage protein |
0.9 (1.9) |
1.0 (2.0) |
NP |
|
|
|
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|
a This list includes only those genes that were upregulated in both the preA and preAB mutant strains overexpressing preA, those confirmed by real-time PCR, genes previously shown to be preA-regulated (yibD, pmrAB) or those known to belong to the PhoPQ or PmrAB regulons b ΔpreA/pBAD18-preA vs. ΔpreA/pBAD18 c ΔpreAB/pBAD18-preA vs. ΔpreAB/pBAD18 d M = Log2(expression plasmid/vector control) e real time PCR (qRT-PCR) performed with cDNA derived from the strains used in Microarray B f NP = not performed g ND = not detected |
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|
Merighi et al. BMC Microbiology 2009 9:42 doi:10.1186/1471-2180-9-42 |
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