L. reuteri strains cultured as planktonic cells produce the antimicrobial compound, reuterin. Stationary phase planktonic cultures of L. reuteri were incubated anaerobically in a glycerol solution. Reuterin concentrations of the cell free supernatants were determined using a colorimetric assay and were normalized with respect to viable colony counts prior to the addition of glycerol. ATCC PTA 6475 and ATCC 5289 produced less reuterin than ATCC 55730 and CF48-3A (ANOVA, p < 0.05).
Jones and Versalovic BMC Microbiology 2009 9:35 doi:10.1186/1471-2180-9-35