Table 1

Primers and probes used in this study.

Gene

Primers or probes

Sequence 5'-3'


mspA

hpor

5'-CGG TCT CAG CGA CCG AAC-3'

npor

5'-CCG GCG ATA CAG TTA GGG A-3'


porM1

mf-4IV-fw

5'-TCT CCA GGG GCT GCT TTT G-3'

mf-4-bw

5'-CGG GAC GCC AAC CAC ATA AC-3'


porM1

komf-3f

5'-CTG AAG CTT CAC CGA GCT GAG CAT CCT CAC-3'

komf-4b

5'-GAC ACT AGT CGT TGG CTA CAG AAC AAC ATT CC-3'


porM2

porM2-51-fw

5'-ATG AAG GCA TTC AGT CGG G-3'

porM2-51-bw

5'-TGC TCC TCA AAG GAG AAG CG-3'


porM2

porM2-rev-1

5'-CGC TTC TCC TTT GAG GAG CA-3'

porM2-rev-2

5'-TCC AGA CCC GCA TGA GAT ACG-3


porM2

porM2-fw-hind

5'-ACA AGC TTC AGC AAC GCT GTG AAC GCA-3'

porM2-bw-hpa

5'-CAG TTA ACA CTA CGG GAC GCT CGT GTC C-3'


porM2

porM2-rna-fw

5'-CGC AAG CCT CTT CGT CGG C-3'

porM2-rna-bw

5'-CCA AGG TGC CCT CGA ACT CAT C-3'


porM1

porM1-as-1

5'-CGG ATC CTA GGG AGA ACA TGA AGG CAT TCA G-3'

porM1-as-2

5'-CGG ATC CTT GTC CAG ACC CGC ATG AGA T-3'


porM1

porM1-fw-hind

5'-ACA AGC TTG CTC TCA GCC GGT TTT CA-3'

porM1-bw-hpa

5'-CAG TTA ACG AAC TGG GCG TTC ATG TGC-3'


porM1

porM1-51-sybr-fw

5'-GCT GTT TAC GAG CAC GGG C-3'

porM1-51-sybr-bw

5'-TTG CGG TCC AGG GGG AAC-3'


mspA

mspATaqFW

5'-CGT GCA GCA GTG GGA CAC CTT-3'

mspATaqBW

5'-CCA CGA TGT ACT TGG CGC GAC-3'

mspATaqProbe

5'-FAM-TGG ACC GCA ACC GTC TTA CCC GTG AGT G-TAMRA-3'


porM

mfpqPCRfw

5'-CGT TCA GCA GTG GGA CAC CTT-3'

mfpqPCRrev

5'-CCA CGG TGT ACT TGG CCC GGC-3'

mfpqPCRprobe

5'-FAM-TGG ACC GCA ACC GGC TGA CCC GTG AGT G-TAMRA-3'


Sharbati et al. BMC Microbiology 2009 9:31   doi:10.1186/1471-2180-9-31

Open Data