Additional file 5.

Knock-down of porins in M. fortuitum 10860/03 by means of anti-sense technology using the plasmid pSRr106. The amount of porM1/porM2 mRNA was quantified by means of qRT-PCR and was normalised with 16S rRNA. Compared to the reference strain M. fortuitum 10860/03 (pSHKLx1) the amount of porM mRNA in the down-regulated strain 10860/03 (pSRr106) was reduced by about 75%.

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Sharbati et al. BMC Microbiology 2009 9:31   doi:10.1186/1471-2180-9-31