Complementation of the porin-deficient mutant strain M. smegmatis ML10 with porM1 and porM2. M. smegmatis ML10 was transformed with the control vector pMV306 (A), the mspA-containing plasmid pSSa100 (B), the porM1-containing plasmid pSRa102 (C) and the porM2-containing plasmid pSRa104 (D). After electroporation of the plasmids, dilutions of the transformed bacteria were plated onto Mycobacteria 7H11 agar with 25 μg ml-1 kanamycin and incubated for four days. Panel (E) illustrates the result of an independent experiment showing the time course of the appearance of the colonies on Mycobacteria 7H11 agar with 25 μg ml-1 kanamycin during four days after plating of a 1:10 dilution of the electroporated cells.
Sharbati et al. BMC Microbiology 2009 9:31 doi:10.1186/1471-2180-9-31