Map of genomic regions containing porM1 from M. fortuitum 10860/03 and porM2 from M. fortuitum 10851/03. Section A shows a 2895 bp region representing the insert of plasmid pSSp107. The insert includes the porM1 gene and three other ORFs. Up- and downstream to porM1 various nucleotide signal sequences were detected: -10 signal of a promoter (TATGTT), a ribosome binding site (RBS: GGAGA), a signal peptide recognition sequence (SP) of 81 bp and a hairpin structure, which could represent a terminator. Furthermore, the location of the antisense fragment selected for the generation of plasmid pSRr106 is indicated. Section B represents a 1697 bp region of M. fortuitum 10851/03 containing porM2 and two other ORFs. Upstream to porM2 a -10 signal of a promoter (TACGTT), a ribosome binding site (AGGGAGAA) and a signal peptide recognition sequence (SP) of 93 bp were identified. Subsequences were predicted using the software packages MacVector™ 7.2.3 (Accelrys) and Lasergene (DNASTAR).
Sharbati et al. BMC Microbiology 2009 9:31 doi:10.1186/1471-2180-9-31