Table 2

Hybridization of well-studied EAEC and DAEC strains to EAEC probes and daaC and results of PCR-RFLP test for daaD and aafB.

Strain

Serotype

Country of isolation/source

HEp-2 adherence pattern*

pAA (CVD 432)

aap

aggA

aafA

daaC

hybrid-ization (SLM 862)

aafB/daaD RFLP

AA 60A

Mexico

Aggregative

+

+

+

-

-

-

AA H232-1

Peru

Aggregative-detaching

+

+

-

-

-

-

AA 17-2

O3:H2

Chile

Aggregative-detaching

+

+

+

-

-

-

AA 253-1

O3:H2

Thailand

Aggregative

+

+

+

-

-

-

AA 6-1

OR:H2

Thailand

Aggregative

+

+

-

-

-

-

BM369

O86

India

Aggregative

-

-

-

-

-

-

AADS65-R2

Philippines

Weak localised-aggregative

-

-

-

-

-

-

AA 501-1

OR:H53

Thailand

Aggregative

-

-

-

-

-

-

AA H223-1

Peru

Aggregative

+

+

-

-

-

-

AA DS67-R2

Philippines

Aggregative

+

+

+

-

-

-

AA 042

O44:H18

Peru

Aggregative

+

+

-

+

+

aafB

AA 144-1

O77:NM

Thailand

Aggregative-detaching

+

+

-

-

+

aafB

AA 44-1

O36:H18

Thailand

Aggregative

+

+

-

-

-

-

AA H145-1

Peru

Aggregative

+

+

+

-

-

-

AA 309-1

O130:H27

Thailand

Aggregative

+

+

+

-

+

aafB

H133

Peru

Aggregative

+

-

-

-

-

-

MH46-2

Peru

Aggregative

+

+

-

-

-

-

M32-1

Peru

Aggregative

-

+

-

-

-

-

C04

Nigeria

Aggregative

+

+

-

-

-

-

C08

Nigeria

Aggregative

+

+

-

-

-

-

AA 103-1

O148:H28

Thailand

Aggregative

+

+

-

-

-

-

AA 435-1

O33:H16

Thailand

Aggregative

+

+

-

+

+

aafB

AA 199-1

OR:H1

Thailand

Aggregative

+

+

-

+

+

aafB

AA H194-2

Peru

Aggregative

+

+

+

-

-

-

AA 278-1

O125ac:H21

Thailand

Aggregative

+

+

-

-

-

-

AA 239-1

OR:H21

Thailand

Aggregative

+

-

-

-

-

-

AA 101-1

O?:H10

Japan

Aggregative

-

-

-

+

+

aafB

G02a

Nigeria

Aggregative-detaching

-

-

+

-

-

-

D163

UK

Aggregative

-

+

-

-

-

-

AA H92-1

Peru

Aggregative

-

-

-

-

-

-

DH5α

CVD

Non-adherent

-

-

-

-

-

-

MG1655

CVD

Non-adherent

-

-

-

-

-

-

DAEC1

CVD

Diffuse

-

-

-

-

+

daaD2

DA57-1186

CVD

Diffuse

-

-

-

-

+

daaD

DA55-2186

CVD

Diffuse

-

-

-

-

+

daaD

DAEC4

CVD

Diffuse

-

-

-

-

+

daaD

TW6350

O157:H45

T. Whittam

Diffuse

-

-

-

-

+

daaD2

DAWC21211

?O81:NM

Thailand

Diffuse

-

-

-

-

-

-


* HEp-2 adherence patterns determined by the 3h assay described by Vial et al. [16]

CVD = Center for Vaccine Development stocks, University of Maryland, Courtesy of JP Nataro

Snelling et al. BMC Microbiology 2009 9:269   doi:10.1186/1471-2180-9-269

Open Data