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Open Access Highly Accessed Research article

Pseudomonas aeruginosa vesicles associate with and are internalized by human lung epithelial cells

Susanne J Bauman and Meta J Kuehn*

Author Affiliations

Duke University Medical Center, Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA

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BMC Microbiology 2009, 9:26  doi:10.1186/1471-2180-9-26

Published: 3 February 2009

Additional files

Additional file 1:

Vesicles primarily colocalize with CT and transferrin in peri-nuclear regions. The data show fluorescently labeled S470 vesicles colocalize with CT and transferrin in perinuclear regions of A549 cells.

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Additional file 2:

PaAP contributes to the cell association of vesicles in a dose-dependent manner. The data show the amount of PaAP on vesicles correlates with the amount of vesicle association with A549 cells.

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Additional file 3:

Vesicle expression and activity of S470APKO5 complemented with plasmid-expressed PaAP. The data show the lack of PaAP activity in the APKO5 strain, the correlation between secreted aminopeptidase activity of the complemented strain with the amount of PaAP secreted, and that induced, plasmid-expressed PaAP in APKO5 is secreted to the same extent as S470 but is not vesicle-associated.

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Open Data