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Open Access Highly Accessed Research article

Gene expression analysis of the biocontrol fungus Trichoderma harzianum in the presence of tomato plants, chitin, or glucose using a high-density oligonucleotide microarray

Ilanit Samolski1, Alberto de Luis2, Juan Antonio Vizcaíno3, Enrique Monte1 and M Belén Suárez14*

  • * Corresponding author: M Belén Suárez belensu@usal.es

  • † Equal contributors

Author Affiliations

1 Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca. Campus de Villamayor-Parque Científico, 37185 Villamayor, Salamanca, Spain

2 Centro de Investigación Biomédica de La Rioja (CIBIR). Piqueras 98, 26006 Logroño, La Rioja, Spain

3 EMBL Outstation, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

4 Current address: Instituto de Microbiología Bioquímica, CSIC/Universidad de Salamanca. Campus Miguel de Unamuno, 37007 Salamanca, Spain

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BMC Microbiology 2009, 9:217  doi:10.1186/1471-2180-9-217

Published: 13 October 2009

Abstract

Background

It has recently been shown that the Trichoderma fungal species used for biocontrol of plant diseases are capable of interacting with plant roots directly, behaving as symbiotic microorganisms. With a view to providing further information at transcriptomic level about the early response of Trichoderma to a host plant, we developed a high-density oligonucleotide (HDO) microarray encompassing 14,081 Expressed Sequence Tag (EST)-based transcripts from eight Trichoderma spp. and 9,121 genome-derived transcripts of T. reesei, and we have used this microarray to examine the gene expression of T. harzianum either alone or in the presence of tomato plants, chitin, or glucose.

Results

Global microarray analysis revealed 1,617 probe sets showing differential expression in T. harzianum mycelia under at least one of the culture conditions tested as compared with one another. Hierarchical clustering and heat map representation showed that the expression patterns obtained in glucose medium clustered separately from the expression patterns observed in the presence of tomato plants and chitin. Annotations using the Blast2GO suite identified 85 of the 257 transcripts whose probe sets afforded up-regulated expression in response to tomato plants. Some of these transcripts were predicted to encode proteins related to Trichoderma-host (fungus or plant) associations, such as Sm1/Elp1 protein, proteases P6281 and PRA1, enchochitinase CHIT42, or QID74 protein, although previously uncharacterized genes were also identified, including those responsible for the possible biosynthesis of nitric oxide, xenobiotic detoxification, mycelium development, or those related to the formation of infection structures in plant tissues.

Conclusion

The effectiveness of the Trichoderma HDO microarray to detect different gene responses under different growth conditions in the fungus T. harzianum strongly indicates that this tool should be useful for further assays that include different stages of plant colonization, as well as for expression studies in other Trichoderma spp. represented on it. Using this microarray, we have been able to define a number of genes probably involved in the transcriptional response of T. harzianum within the first hours of contact with tomato plant roots, which may provide new insights into the mechanisms and roles of this fungus in the Trichoderma-plant interaction.