Table 2

Strains and plasmids

Strain or plasmid

Relevant characteristics

Source and/or reference


B. cenocepacia strains


J2315

CF clinical isolate

G. Manno

D1

J2315 ΔBCAS0591-BCAS0593

This study

D3

J2315 ΔBCAL1672-BCAL1676

This study

D4

J2315 ΔBCAL2820-BCAL2822

This study


E. coli strains


DH5α

F- Φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK- mK+) supE44 thi-1 ΔgyrA96 relA1

Laboratory stock

SY327

araD Δ(lac pro) argE(Am) recA56 nalA λ pir; Rifr

[43]


Plasmids


pGEM-T Easy

Vector for PCR cloning, Ampr

Promega

pGPISce-I

oriR6K, ΩTpr, mob+, containing the ISce-I restriction site

[32]

pRK2013

oricolE1, RK2 derivative, Kanr, mob+, tra+

[44]

pDAISce-I

pDA12 encoding the ISce-I homing endonuclease

[32]

pOP1/pGPI-SceI

Plasmid for construction of D1 deletion mutant

This study

pOP3/pGPI-SceI

Plasmid for construction of D3 deletion mutant

This study

pOP4/pGPI-SceI

Plasmid for construction of D4 deletion mutant

This study

pSCR1

Ampr, pQF50 containing PcepI-lacZ and cepR

[42]


Ampr, ampicillin resistance; Kanr, kanamycin resistance; Rifr, rifampin resistance; Tetr, tetracycline resistance; Tpr, trimethoprim resistance.

Buroni et al. BMC Microbiology 2009 9:200   doi:10.1186/1471-2180-9-200

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