Additional file 7.
Design of a homologous IPC for use in the qPCR/MCA or qPCR assays. The IPC monitored by a characteristic melting temperature or by an alternatively labeled hydrolysis probe in the qPCR/MCA or qPCR assays, respectively, helps to prevent false-negative detection due to insufficient extraction and/or amplification.
Format: PDF Size: 117KB Download file
This file can be viewed with: Adobe Acrobat Reader
Hochwimmer et al. BMC Microbiology 2009 9:184 doi:10.1186/1471-2180-9-184