Additional file 7.

Design of a homologous IPC for use in the qPCR/MCA or qPCR assays. The IPC monitored by a characteristic melting temperature or by an alternatively labeled hydrolysis probe in the qPCR/MCA or qPCR assays, respectively, helps to prevent false-negative detection due to insufficient extraction and/or amplification.

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Hochwimmer et al. BMC Microbiology 2009 9:184   doi:10.1186/1471-2180-9-184