Table 4

Organic acid production by fluorescent Pseudomonas during Mussoorie rock phosphate solubilization.

Organic acid (μg/ml)


Strain

P-liberated (μg/ml)

Final pH

Oxalic

Gluconic

2-KGA

Lactic

Succinic

Formic

Citric

Malic

Total organic acids (μg/ml)


P. trivialis

BIHB 728

11.0 ± 0.3

3.52

15.1 ± 1.4

8443.3 ± 6.0

ND

44.9 ± 1.7

ND

ND

ND

ND

8503.3

BIHB 736

13.1 ± 0.1

3.52

15.6 ± 1.4

9314.3 ± 7.4

ND

ND

ND

ND

ND

ND

9329.9

BIHB 745

5.8 ± 0.3

3.63

14.8 ± 1.4

9394.0 ± 8.3

ND

ND

ND

84.0 ± 3.1

ND

930.0 ± 4.2

10422.8

BIHB 747

12.0 ± 0.2

3.49

16.3 ± 0.7

10016.7 ± 4.4

ND

36.8 ± 2.0

ND

70.4 ± 2.7

ND

ND

10140.2

BIHB 749

8.0 ± 0.04

3.59

15.8 ± 0.7

12027.0 ± 5.7

ND

ND

ND

ND

ND

ND

12042.8

BIHB 750

4.8 ± 0.4

3.67

11.7 ± 0.9

8460.0 ± 5.8

ND

ND

ND

ND

ND

32.3 ± 2.1

8504.0

BIHB 757

9.0 ± 0.04

3.63

10.6 ± 1.0

9460.0 ± 5.5

ND

39.4 ± 2.8

ND

ND

ND

ND

9510.0

BIHB 759

11.0 ± 0.2

3.52

16.7 ± 1.3

13854.0 ± 4.9

ND

39.7 ± 1.3

ND

ND

ND

ND

13910.4

BIHB 763

12.9 ± 0.02

3.50

18.2 ± 0.5

13444.0 ± 5.5

ND

ND

ND

87.7 ± 3.0

ND

ND

13549.9

BIHB 769

6.1 ± 0.4

3.65

16.4 ± 0.7

11633.7 ± 5.4

ND

40.5 ± 2.6

ND

ND

ND

ND

11690.6

P. poae

BIHB 730

4.0 ± 0.06

4.62

12.5 ± 1.3

7871.0 ± 8.5

19.9 ± 1.4

37.8 ± 2.1

ND

ND

ND

ND

7941.2

BIHB 752

6.0 ± 0.03

3.62

19.6 ± 2.1

15727.0 ± 5.9

ND

ND

ND

ND

ND

293.0 ± 4.7

16039.6

BIHB 808

8.6 ± 0.6

3.53

15.3 ± 1.2

13749.7 ± 3.4

ND

ND

ND

ND

ND

ND

13765.0

P. fluorescens

BIHB 740

3.0 ± 0.1

5.90

14.3 ± 0.9

8051.0 ± 6.1

468.0 ± 3.1

ND

ND

114.4 ± 4.9

ND

183.2 ± 4.9

8830.9

Pseudomonas spp.

BIHB 751

2.4 ± 0.1

3.89

11.7 ± 0.4

7076.3 ± 4.6

126.3 ± 7.2

ND

ND

ND

ND

2802.0 ± 4.7

10016.3

BIHB 756

12.7 ± 0.4

3.53

14.7 ± 1.2

9120.0 ± 6.4

153.0 ± 3.1

ND

142.0 ± 3.5

ND

ND

264.0 ± 4.6

9693.7

BIHB 804

8.1 ± 0.3

3.55

39.3 ± 1.5

8997.0 ± 7.2

18.4 ± 0.9

39.6 ± 1.1

ND

ND

ND

34.1 ± 2.9

9128.4

BIHB 811

2.9 ± 0.03

4.00

42.0 ± 1.7

10007.0 ± 3.8

234.3 ± 2.0

50.8 ± 2.3

349.7 ± 2.7

ND

22.3 ± 2.2

36.1 ± 2.8

10742.2

BIHB 813

2.2 ± 0.4

4.05

14.2 ± 0.7

10396.0 ± 5.6

ND

40.5 ± 2.0

136.0 ± 2.1

ND

ND

ND

10586.7


Total organic acids (μg/ml)

334.8

197042.0

1019.9

370.0

627.7

356.5

22.3

4574.7

204347.9


Values are the mean of three replicates ± standard error of the mean; ND = Not detected; 2-KGA = 2-ketogluconic acid.

Vyas and Gulati BMC Microbiology 2009 9:174   doi:10.1186/1471-2180-9-174

Open Data