Table 2

MIC results and Erg11p substitutions for 25 C. albicans isolates with reduced susceptibility to fluconazole and 23 fluconazole-susceptible isolates by RCAa and ERG11 sequencing.

MIC (μg/ml)

Erg 11p amino acid substitutions

D

D

E

F

F

G

G

G

G

K

K

R

S

V

V

Y

1

2

2

1

4

3

4

4

4

1

1

4

4

4

4

1

Patient/isolate no.

Site

FLUa

VORa

1

7

6

4

4

0

4

6

6

2

4

6

0

3

8

3

6

8

6

5

9

7

8

4

5

8

3

7

5

7

8

2

E

E

D

L

S

S

E

S

S

T

R

K

F

I

I

H


Isolates with reduced fluconazole susceptibility

1b

Oropharynx

16

0.25

+

+

+

2b

Vagina

>256

0.03

3-Ab, c

Oropharynx

16

0.25

+

+

+

- Bb, c

Oropharynx

16

0.5

+

+

+

4d

Oropharynx

256

0.25

+

+

+

5d

Oropharynx

256

0.125

+

6-Ac, d

Oropharynx

256

>16

+

-Bc, d

Oropharynx

256

>16

+

7d

Oropharynx

256

>16

+

+

+

+

8-Ac, d

Oropharynx

256

0.5

+

+

+

-Bc, d

Oropharynx

256

1

+

+

+

9d

Oropharynx

256

>16

+

+

10d

Oropharynx

256

2

+

+

+

+

11d

Oropharynx

256

0.008

+

+

12-Ac, d

Oropharynx

256

2

+

-Bc, d

Sputum

32

0.5

+

+

+

13d

Vagina

32

0.016

+

14d

Blood

256

>16

+

+

15d

Bile

256

16

+

16-Ac, d

Oropharynx

16

0.125

+

+

+

-Bc

Oropharynx

256

2

+

+

+

+

-Cc, d

Oropharynx

256

16

+

+

+

+

+

17d

Oropharynx

64

0.5

+

18d

Oropharynx

128

2

+

+

+

19d

Oropharynx

256

0.5

+

+

+

Fluconazole-susceptible isolatesb

ATCC 10231

UNa

0.125

0.008

ATCC 90028

Blood

025

0.03

20

Blood

0.25

<0.008

+

+

21

Oropharynx

0.12

0.008

+

+

22

Blood

0.5

0.016

23

Blood

0.5

0.016

24

Blood

0.25

0.008

25

Blood

0.25

0.008

+

+

26

Blood

0.5

0.008

+

+

27

Blood

0.5

0.008

+

+

28

Blood

0.25

<0.008

+

+

29

Skin

1

0.016

+

+

30

Peritoneal fluid

1

2

+

+

31

Blood

0.12

0.125

+

+

+

32

Blood

0.125

0.008

+

+

33

Blood

0.5

0.008

+

+

+

34

Tissue

0.25

0.008

+

+

35

Blood

2

0.008

+

+

36

Blood

0.25

0.016

+

+

37

Liver

0.5

<0.008

+

+

38

Blood

0.25

<0.008

+

+

+

39

Blood

0.125

<0.008

+

40

Bone

0.25

<0.008

+

+


The "+" sign denotes the presence of the mutation.

aAbbreviations: RCA, rolling circle amplification; FLU, fluconazole; VOR, voriconazole; UN, unknown.

b Isolates from the Centre for Infectious Diseases and Microbiology, Westmead Hospital, Sydney.

c The "A" and "B" notation of patient numbers refers to isolates cultured sequentially from the same patient at different times.

dIsolates obtained from the Mycology Unit, Women's and Children's Hospital, Adelaide.

Wang et al. BMC Microbiology 2009 9:167   doi:10.1186/1471-2180-9-167

Open Data