Figure 1.

Comparison of the amplification efficacy between the gyrB/parE primer pairs of this study (lanes 1, 3, 5, and 7) and those of Roth et al., (2004) [4] (lanes 2, 4, 6, and 8). The production of ssDNA during the PCR program are shown with the species of E. faecalis (lane 1 and 2), E. faecium (lane 3 and 4). K. pneumoniae (lane 5 and 6), and N. meningitidis (lane 7 and 8) by gel electrophoresis using a 2% agarose gel containing SYBR® Green II. The ssDNA amplicons of gyrB/parE (200 bp) were detected using the primer pair of this study together with the dsDNA amplicons of gyrB/parE (300 bp).

Järvinen et al. BMC Microbiology 2009 9:161   doi:10.1186/1471-2180-9-161
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