Table 3 |
||
|
Detection limits of luminescent Salmonella typhimurium. |
||
|
Item |
Bacterial concentration (CFU) |
Photonic emissions (RLU/s) |
|
|
||
|
Black tube format (1ml) upper limit (2 s acquisition time) |
||
|
Background (used for subtraction of sample) |
- |
39 |
|
pAK1-lux |
1.1 × 108 ± 1.0 × 107 |
7,470 ± 136 |
|
pCGLS-1 |
6.2 × 107 ± 1.2 × 107 |
6,168 ± 167 |
|
pXEN-1 |
1.0 × 108 ± 1.0 × 107 |
7,016 ± 136 |
|
Black tube format (1. 0 ml) lower limit (2 s acquisition time) |
||
|
Background (used for subtraction of sample) |
- |
33 |
|
pAK1-lux |
2.7 × 106 ± 1.0 × 107 |
278 ± 136 |
|
pCGLS-1 |
1.8 × 106 ± 1.0 × 107 |
327 ± 136 |
|
pXEN-1 |
5.1 × 106 ± 1.0 × 107 |
148 ± 141 |
|
|
||
|
Item |
Bacterial concentration (CFU) |
Photonic emissions (RLU/s) |
|
|
||
|
96-well black plate format (100 μl) lower limit (30 s acquisition time) |
||
|
Background (used for subtraction of sample) |
- |
6 |
|
pAK1-lux |
3.8 × 103 ± 2.8 × 103 |
2.0 ± 1.3 |
|
pCGLS-1 |
2.9 × 103 ± 2.8 × 103 |
1.1 ± 1.3 |
|
pXEN-1 |
2.8 × 103 ± 2.7 × 103 |
1.1 ± 1.2 |
|
|
||
|
Luminescent Salmonella typhimurium with three different plasmids (pAK1-lux, pXEN-1, and pCGLS-1); upper and lower detection limits for black tube format (2 s acquisition time) and low detection limits for black 96-well plate format (30 s acquisition time). The results below are bacterial concentration means ± standard error of the mean and photonic emissions means ± standard error of the mean. |
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|
Moulton et al. BMC Microbiology 2009 9:152 doi:10.1186/1471-2180-9-152 |
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