Table 1 

Composition of the human microbiota compared in three age groups 

TaqMan detection 
SYBRGreen detection 




Firmicutes 
Firmicutes 
Firmicutes 




n 
Allbacteria (a) 
C. leptum group (b) 
C. coccoides group (b) 
Bacteroides/Prevotella group (b) 
Bifidobacterium genus (b) 
E. coli (b) 
Lactobacillus/Leuconostoc/Pediococcus group (b) 



Infant 
21 
10.7 ± 0.1 (A) 
3.2 ± 0.4 (A) 
3.2 ± 0.4 (A) 
1.5 ± 0.3 (A) 
0.6 ± 0.2 (A) 
1.5 ± 0.3 (A) 
3 ± 0.2 (A) 


Adult 
21 
11.5 ± 0.1 (B) 
0.7 ± 0.1 (B) 
1.2 ± 0.1 (B) 
1.5 ± 0.1 (AB) 
2.3 ± 0.3 (B) 
3.8 ± 0.1 (B) 
3.9 ± 0.3 (AB) 


Elder 
20 
11.4 ± 0.1 (B) 
1.1 ± 0.1 (C) 
1.8 ± 0.1 (A) 
1 ± 0.1 (A) 
2.3 ± 0.3 (B) 
2.4 ± 0.2 (C) 
4.2 ± 0.2 (B) 


n represents the number of samples in each group. (a) Allbacteria results obtained by qPCR were expressed as the mean of the log_{10 }value ± SEM. (b) Results were expressed as the mean of the log_{10 }value ± SEM of normalized data calculated as the log of targeted bacteria minus the log of Allbacteria number. The non parametric Wilcoxon test was performed. Data not sharing the same letter within a column are significantly diferrent at p < 0.05. 

Mariat et al. BMC Microbiology 2009 9:123 doi:10.1186/147121809123 