Table 1

Composition of the human microbiota compared in three age groups

TaqMan detection

SYBR-Green detection

Firmicutes

Firmicutes

Firmicutes

n

All-bacteria (a)

C. leptum group (b)

C. coccoides group (b)

Bacteroides/Prevotella group (b)

Bifidobacterium genus (b)

E. coli (b)

Lactobacillus/Leuconostoc/Pediococcus group (b)

Infant

21

10.7 ± 0.1 (A)

-3.2 ± 0.4 (A)

-3.2 ± 0.4 (A)

-1.5 ± 0.3 (A)

-0.6 ± 0.2 (A)

-1.5 ± 0.3 (A)

-3 ± 0.2 (A)

Adult

21

11.5 ± 0.1 (B)

-0.7 ± 0.1 (B)

-1.2 ± 0.1 (B)

-1.5 ± 0.1 (AB)

-2.3 ± 0.3 (B)

-3.8 ± 0.1 (B)

-3.9 ± 0.3 (AB)

Elder

20

11.4 ± 0.1 (B)

-1.1 ± 0.1 (C)

-1.8 ± 0.1 (A)

-1 ± 0.1 (A)

-2.3 ± 0.3 (B)

-2.4 ± 0.2 (C)

-4.2 ± 0.2 (B)

n represents the number of samples in each group.

(a) All-bacteria results obtained by qPCR were expressed as the mean of the log₁₀ value ± SEM.

(b) Results were expressed as the mean of the log₁₀ value ± SEM of normalized data calculated as the log of targeted bacteria minus the log of All-bacteria number.

The non parametric Wilcoxon test was performed.

Data not sharing the same letter within a column are significantly diferrent at p < 0.05.

Mariat et al. BMC Microbiology 2009 9:123   doi:10.1186/1471-2180-9-123