Table 2

Bacterial strains and plasmids used in this study

Bacterial strains and plasmids

Genotypes

(references)


E. coli

N3431

rne-3071ts, lacZ43, LAM-, relA1, spoT1 (CGSC#6975)

[36]

S. sonnei

HW383

S. sonnei wild-type strain, (Tcr)

[7]

HW506

S. sonnei HW383 without pSS120 plasmid (Tcr, non invasive)

[7]

MS506

HW506 (Tcs)

This study

MS390

HW383 (Tcs)

[13]

MS1632

MS390ΔinvE

[11]

MS2830

MS390ΔcpxR (cpxR: chromosomal activator of virF gene)

[13]

MS4831

MS390Δhfq

[11]

MS4841

MS390Δhns (non invasive)

[11]

MS5400

MS390Δrne701–892::aphA

This study

MS5512

MS390ΔpinvE::paraBAD

[11]

S. flexneri

2457T

S. flexneri 2a wild-type strain,

[49]

2457O

2457T carrying mutation in virF gene (non-invasive)

[50]

MF4835

2457TΔhfq::aphA

[11]

Plasmids

pBAD-invE

PCR-amplified invE gene was cloned into pBAD24 (Apr)

[11]

pHW848

virF-lacZ translational fusion plasmid (Cmr)

[8]

pJK1142

invE and ipa-mxi-spa (TTSS) genes encoding plasmid (Kmr)

[4]

pJK1143

virF-encoding plasmid (Cmr)

[4]

pJM4320

invE-lacZYA transcriptional fusion in pTH18cs5(Cmr)

[13]

pJM4321

invE-lacZYA translational fusion in pTH18cs5(Cmr)

[13]

pTrc99A

IPTG inducible expression plasmid(Apr)

[51]

pTrc-hfq

PCR-amplified hfq gene was cloned into pTrc99A(Apr)

[11]


Mitobe et al. BMC Microbiology 2009 9:110   doi:10.1186/1471-2180-9-110

Open Data