|
Extent of phagocytosis of A. fumigatus and P. chrysogenum conidia by HNEC revealed using the fluorescence brightener Blankophor to stain non-phagocytosed conidia. |
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| Contact time |
||||||
| HNEC culture |
Fungal species |
Bound conidia (%) |
4 h |
8 h |
12 h |
20 h |
|
|
||||||
| C313P |
A. fumigatus |
12.2 |
18.4 |
33.4 |
Nd |
Nd |
| C321P |
A. fumigatus |
14.4 |
8.3 |
12.5 |
nd |
nd |
| C338P |
A. fumigatus |
12.0 |
12.8 |
28.6 |
nd |
nd |
| C349P |
A. fumigatus |
9.9 |
nd |
14.5 |
19.2 |
38.1 |
| C350P |
A. fumigatus |
9.5 |
nd |
20.9 |
16.1 |
27.6 |
| C360Pa |
A. fumigatus |
8.6 |
nd |
11.5 |
9.9 |
20.9 |
| C367Pa |
A. fumigatus |
7.5 |
nd |
9.2 |
9.7 |
20.3 |
| Mean |
10.6 ± 2.4 |
13.2 ± 5.1 |
18.7 ± 9.3 |
14.0 ± 4.7 |
26.7 ± 8.3 |
|
| C360Pa |
P. chrysogenum |
8.9 |
nd |
9.0 |
11.9 |
17.2 |
| C367Pa |
P. chrysogenum |
6.4 |
nd |
10.2 |
10.5 |
18.3 |
| Mean |
7.7 |
9.6 |
11.2 |
17.8 |
||
|
Percentages and standard deviations were calculated from the bound conidia figures for two wells at each time point with seven and two different primary cultures for A. fumigatus and P. chrysogenum respectively. nd: not done ap value > 0.05 with the non-parametric Kruskall Wallis test for the two species for the same primary culture. | ||||||
Botterel et al. BMC Microbiology 2008 8:97 doi:10.1186/1471-2180-8-97 |
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