Development of multiple strain competitive index assays for Listeria monocytogenes using pIMC; a new site-specific integrative vector

Ian R Monk¹^,2 , Pat G Casey² , Michael Cronin³ , Cormac GM Gahan¹^,2^,4 and Colin Hill¹^,2

¹Department of Microbiology, University College Cork, Cork, Ireland

²Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland

³Department of Maths and Statistics, University College Cork, Cork, Ireland

⁴Department of Pharmacy, University College Cork, Cork, Ireland

author email corresponding author email

BMC Microbiology 2008, 8:96doi:10.1186/1471-2180-8-96


Published:	13 June 2008

Additional files

Additional file 1:

Statistical analysis (One Sample T-test) of raw CFU. Attached is a Microsoft Excel spreadsheet for the statistical analysis from organ counts (e.g. spleen and liver). The raw CFU count from each strain per organ is inserted in the indicated boxes and the statistical analysis output is on the right hand side.

Format: XLS Size: 54KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

Calculation and statistical analysis (One Sample T-test) of the relative virulence ratio (RVR). Attached is a Microsoft Excel spreadsheet for the calculation and statistical analysis from organ counts (E.g. spleen and liver) converted into RVR. The raw CFU count from each strain per organ is inserted in the indicated boxes and the statistical analysis output from the RVR is on the right hand side.

Format: XLS Size: 65KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Conversion of CFU to competitive index relative to strain 1. Attached is a Microsoft Excel spreadsheet for the conversion of organ counts (E.g. spleen and liver) to the RVR and subsequent calculation of competitive index relative to strain 1. The raw CFU count from each strain per organ is inserted in the indicated boxes and the CI calculated on the right hand side.

Format: XLS Size: 53KB Download file

This file can be viewed with: Microsoft Excel Viewer