Dxr is essential in Mycobacterium tuberculosis and fosmidomycin resistance is due to a lack of uptake

doi:10.1186/1471-2180-8-78

BMC Microbiology

Volume 8

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Research article

Dxr is essential in Mycobacterium tuberculosis and fosmidomycin resistance is due to a lack of uptake

Amanda C Brown and Tanya Parish

Institute for Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK

author email corresponding author email

BMC Microbiology 2008, 8:78doi:10.1186/1471-2180-8-78


Published:	20 May 2008

Additional files

Additional file 1:

Schematic representation of the bioassay used to quantify fosmidomycin. Cells were exposed to fosmidomycin; cell-free extracts or culture filtrates were prepared and applied to sterile paper discs. The discs were aseptically placed on media containing E. coli, as a test organism. The diameter of the zone of growth inhibition was measured following incubation. Using known amounts of fosmidomycin a standard curve of growth inhibition was constructed and used to quantify the fosmidomycin in the samples.

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