Methodology article
Stable transformation of an episomal protein-tagging shuttle vector in the piscine diplomonad Spironucleus vortens
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* Corresponding author: Scott C Dawson scdawson@ucdavis.edu
1 Department of Microbiology, 255 Briggs Hall, One Shields Ave., UC-Davis Davis, CA 95616, USA
2 Molecular and Cell Biology, 345 Life Science Addition, UC-Berkeley, Berkeley, CA 94720-3200, USA
BMC Microbiology 2008, 8:71 doi:10.1186/1471-2180-8-71
Published: 29 April 2008Additional files
Additional file 1:
Live time-lapse epifluorescent microscopy movie of SvH3P strain. Live image analysis of the SvH3P strain using epifluorescent microcopy. This short movie corresponds to the still image presented in Figure 3A, and demonstrates the cytoplasmic native GFP expression in the transformed S. vortens SVH3P strain. This movie demonstrates live GFP-tagged S. vortens and highlights the cytoplasmic localization of green fluorescent protein (GFP) expressed from the H3 histone promoter. This short movie corresponds to the still image presented in Figure 3A
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Additional file 2:
Live 3D movie of the SvH3G strain. Live image analysis of the SvH3G strain using epifluorescent microcopy. This short three-dimensional stack presented as a movie corresponds to the still image presented in Figure 4A, and demonstrates the native GFP expression in both nuclei in the transformed SvH3G S. vortens strain. This movie shows the live histone H3 GFP-tagged S. vortens strain and highlights the nuclear localization of the H3G:GFP fusion. This short movie corresponds to the still image presented in Figure 4A
Format: QT Size: 213KB Download file