Figure 3.

Illustration of the specificity of the hybridization reaction on the AT microarray for genotyping of C. psittaci. Alignments of target (ompA gene segments) and probe sequences are shown on the left-hand side, and the respective hybridization signals (including internal staining marker) are given on the right-hand side. Upper part: The signal generated by duplex formation at genotype E-specific probe VD2-04 is reduced to approx. 20% when the target has a single mismatch, such as genotype B. This applies also to genotype B-specific probe VD2-03, when reacting with genotype E. Lower part: Signal is reduced to less than 10% in the case of two mismatches on the target sequence.