Table 1

Bacterial strains, plasmids and antibodies used in this study

Strain, plasmid or antibody

Relevant characteristics

Reference or source


Strains

S. parasanguinis

FW213

Wild type

[7]

VT1393

fap1::aphA-3, null mutant of fap1, KanR

[8]

VT1619

gap3 replacement mutant, KanR

[23]

VT1731

pVT1666 into VT1619, vector control strain, ErmR & KanR

This study

VT1732

pVT1732 into VT1619, gap3 complemented strain, ErmR &KanR

This study

VT1735

Gap3 aa 62–67 encoding region deletion in pVT1732, ErmR &KanR

This study

VT1737

Gap3 aa 144–157 encoding region deletion in pVT1732, ErmR &KanR

This study

VT1738

Gap3 V33R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1739

Gap3 F35F mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1740

Gap3 N37I mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1741

Gap3 P38R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1742

Gap3 S42L mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1743

Gap3 N54I mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1744

Gap3 P62G mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1745

Gap3 D63V mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1746

Gap3 L64R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1747

Gap3 P65R mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1748

Gap3 I66N mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1749

Gap3 L67T mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1750

Gap3 L75S mutation of pVT1732 in VT1619, ErmR &KanR

This study

VT1751

Gap3 R59L mutation of pVT1732 in VT1619, ErmR &KanR

This study

E. coli

DH10B

Host strain for cloning

Invitrogen

XL-10-Gold

Host strain for site-directed mutagenesis

Stratagene

Plasmids

pVT1571

An E. coli and S. parasanguinis shuttle vector, parent of pVPT1686

[11]

pVT1666

PVT1571 vector contained gfp gene

This study

pVT1732

pVT1666 contained gap3 gene

This study

Antibodies

D10

Partilly glycosylated Fap1 glycan epitope-specific MAb

[20]

E42

Fap1 peptide epitope-specific MAb

[20]

F51

Mature Fap1 glycan epitope-specific MAb

[20]

Anti-GFP

MAb against GFP tag

Roche Company


Peng et al. BMC Microbiology 2008 8:52   doi:10.1186/1471-2180-8-52

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