Table 2

Results for 96 NTM isolates for which phenotypic methods gave incorrect species identification as determined by hsp65 sequencing.

Species

Nb

Phenotypic result


M. abscessus (1)c

1

SGN

M. arupense (5)

1

M. chelonae complex

2

SGN

1

SGS

M. asiaticum (3)

2

M. avium complex

1

M. gordonae

M. avium (18)

1

M. chelonae complex

1

M. fortuitum

2

M. kansasii

10

SGN

1

RGN

1

SGP

1

SGS

M. celatum (2)

1

M. xenopi

1

SGN

M. chelonae (2)

2

SGN

M. cosmeticum (1)

1

M. chelonae

M. farcinogenes (1)

1

M. chelonae complex

M. flavescens (1)

1

RGS

M. fortuitum (6)

2

M. chelonae complex

1

RGN

1

SGN

M. genavense (1)

1

SGN

M. gordonae (26)

1

RGP

1

SGN

12

SGS

M. hassiacum (1)

1

RGS

M. intracellulare (9)

1

M. chelonae complex,

1

M. gordonae

7

SGN

M. kansasii (7)

1

M. nonchromogenicum

2

RGP

1

SGN

2

SGP

1

SGS

M. lentiflavum (3)

2

M. avium complex

1

M. gordonae

M. mageritense (1)

1

M. fortuitum

M. marinum (1)

1

M. kansasii

M. mucogenicum (8)

2

M. chelonae complex

1

M. fortuitum complex

1

M. peregrinum

3

SGN

1

SGS

M. nebraskense (1)

1

M. gordonae

M. nonchromogenicum (2)

1

SGN

M. peregrinum (4)

4

M. chelonae complex

M. phlei (1)

1

RGS

M. scrofulaceum (3)

1

M. avium complex

2

SGN

M. sherrisii (3)

2

M. avium complex

1

SGN

M. szulgai (1)

1

SGS

M. terrae (2)

1

SGN


a Species identification was determined by hsp65 sequencing for 113 isolates that had discordant results by PRA-hsp65 and phenotypic studies. For 17 isolates sequencing confirmed the species identification obtained by phenotypic methods.

b Number of isolates for which the phenotypic identification shown was incorrect.

c Total number of isolates of that species sequenced. SGS: slowly growing scotochromogen; SGN: slowly growing nonchromogen; SGP: slowly growing photochromogen; RGS: rapidly growing scotochromogen; RGN: rapidly growing nonchromogen; RGP: rapidly growing photochromogen.

Chimara et al. BMC Microbiology 2008 8:48   doi:10.1186/1471-2180-8-48

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