Table 8

Results obtained for the pressure ulcer sample (P) using shotgun Sanger sequencing (FRACS).

Genus species

Seq

%

Gram

Aerotolerance

Shape


Peptoniphilus ivorii

51

27.42

+

anaerobic

cocci

Anaerococcus lactolyticus

20

10.75

+

anaerobic

cocci

Anaerococcus vaginalis

16

8.60

+

anaerobic

cocci

Streptococcus dysgalactiae*

14

7.53

+

facultative

cocci

Peptoniphilus harei

14

7.53

+

anaerobic

cocci

Peptococcus niger

12

6.45

+

anaerobic

rod

Serratia marcescens

12

6.45

-

aerobic

rod

Finegoldia magna

10

5.38

+

anaerobic

cocci

Peptoniphilus indolicus

7

3.76

+

anaerobic

cocci

Clostridium hathewayi

4

2.15

+

anaerobic

rod

Prevotella buccalis

3

1.61

-

anaerobic

rod

Pseudomonas aeruginosa*

3

1.61

-

aerobic

rod

UWB

2

1.08

Unk

unk

unk

Dialister invisus

2

1.08

-

anaerobic

rod

Dialister spp.

2

1.08

-

anaerobic

rod

Delftia acidovorans

2

1.08

-

aerobic

rod

Pseudomonas fluorescens*

2

1.08

-

aerobic

rod

Helcococcus kunzii

1

0.54

+

facultative anaerobe

cocci

Enterococcus faecalis*

1

0.54

+

facultative

cocci

Staphylococcus epidermidis*

1

0.54

+

facultative

cocci

Streptococcus pyogenes*

1

0.54

+

facultative

cocci

Clostridium perfringens

1

0.54

+

anaerobic

rod

Klebsiella granulomatis

1

0.54

-

Facultative anaerobe

rod

Klebsiella pneumoniae

1

0.54

-

Facultative anaerobe

rod

Proteus vulgaris*

1

0.54

-

Facultative anaerobe

rod

Porphyromonas uenonis

1

0.54

-

anaerobic

rod

Pseudomonas spp.*

1

0.54

-

aerobic

rod


This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the P sample, the number of sequences corresponding to the given genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the P pool. Bacteria that were cultured and were not found using FRACSS include Acinetobacter, Enterobacter, Leclercia and Morganella. The % represents the percentage of the total sequences analyzed within the sample.

Dowd et al. BMC Microbiology 2008 8:43   doi:10.1186/1471-2180-8-43

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