Table 4

Results obtained for the pressure ulcer sample (P) using pyrosequencing (PRAPS).

Genus

Seq

%

Gram

Aerotolerance

Shape


Peptoniphilus spp.

10543

38.38

+

Anaerobe

Cocci

Serratia spp.

5234

19.05

-

facultative anaerobe

Rod

Peptococcus niger.

3042

11.07

+

anaerobe

Cocci

Streptococcus spp.

3016

10.98

+

facultative anaerobe

Cocci

Finegoldia magna

1743

6.34

+

anaerobe

Cocci

Dialister spp.

1374

5.00

-

anaerobe

rod

Pectobacterium spp.

528

1.92

-

facultative anaerobe

rod

Enterobacter spp.

392

1.43

-

facultative anaerobe

rod

Proteus spp.

308

1.12

-

facultative anaerobe

rod

Veillonella spp.

186

0.68

-

anaerobe

cocci

UWB

141

0.51

Unk

unk

unk

UWB

121

0.44

Unk

unk

unk

Clostridium spp.

93

0.34

+

anaerobe

rod

Corynebacterium striatum

73

0.27

+

aerobe

rod

Delftia spp.

65

0.24

-

aerobe

rod

UWB

63

0.23

Unk

unk

unk

Enterococcus spp.

62

0.23

+

facultative anaerobe

cocci

Staphylococcus spp.

56

0.20

+

facultative anaerobe

cocci

Hydrogenophaga spp.

54

0.20

-

aerobe

rod

Eggerthella lenta

33

0.12

+

anaerobe

rod

UWB

31

0.11

Unk

unk

unk

UWB

31

0.11

Unk

unk

unk

Prevotella spp.

31

0.11

-

anaerobe

rod

Varibaculum cambriense

28

0.10

+

anaerobe

rod

Actinomyces europaeus

28

0.10

+

facultative anaerobe

rod

Ferrimonas spp.

27

0.10

-

facultative anaerobe

rod

Bacillus spp.

24

0.09

+

aerobe

rod

UWB

22

0.08

Unk

unk

unk

Fusobacterium spp.

22

0.08

-

anaerobe

rod

Alcaligenes faecalis

20

0.07

-

aerobe

rod

UWB

17

0.06

Unk

unk

unk

Riemerella spp.

15

0.05

-

aerobe

rod

UWB

14

0.05

Unk

unk

unk

Stenotrophomonas spp.

14

0.05

-

aerobe

rod

Shewanella spp.

11

0.04

-

facultative anaerobe

rod

Eubacterium spp.

10

0.04

+

anaerobe

rod


This table provides the identified genus of the bacteria found using pyrosequencing (PRAPS) in the P sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. * The asterisk identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the P pool. Bacteria that were cultured and were not found using PRAPS include Acinetobacter, Leclercia, Morganella, and Pseudomonas. The % represents the percentage of the total sequences analyzed within the sample.

Dowd et al. BMC Microbiology 2008 8:43   doi:10.1186/1471-2180-8-43

Open Data