Table 4

Results obtained for the pressure ulcer sample (P) using pyrosequencing (PRAPS).

Genus
Seq
%
Gram
Aerotolerance
Shape

Peptoniphilus spp.
10543
38.38
+
Anaerobe
Cocci
Serratia spp.
5234
19.05
-
facultative anaerobe
Rod
Peptococcus niger.
3042
11.07
+
anaerobe
Cocci
Streptococcus spp.
3016
10.98
+
facultative anaerobe
Cocci
Finegoldia magna
1743
6.34
+
anaerobe
Cocci
Dialister spp.
1374
5.00
-
anaerobe
rod
Pectobacterium spp.
528
1.92
-
facultative anaerobe
rod
Enterobacter spp.
392
1.43
-
facultative anaerobe
rod
Proteus spp.
308
1.12
-
facultative anaerobe
rod
Veillonella spp.
186
0.68
-
anaerobe
cocci
UWB
141
0.51
Unk
unk
unk
UWB
121
0.44
Unk
unk
unk
Clostridium spp.
93
0.34
+
anaerobe
rod
Corynebacterium striatum
73
0.27
+
aerobe
rod
Delftia spp.
65
0.24
-
aerobe
rod
UWB
63
0.23
Unk
unk
unk
Enterococcus spp.
62
0.23
+
facultative anaerobe
cocci
Staphylococcus spp.
56
0.20
+
facultative anaerobe
cocci
Hydrogenophaga spp.
54
0.20
-
aerobe
rod
Eggerthella lenta
33
0.12
+
anaerobe
rod
UWB
31
0.11
Unk
unk
unk
UWB
31
0.11
Unk
unk
unk
Prevotella spp.
31
0.11
-
anaerobe
rod
Varibaculum cambriense
28
0.10
+
anaerobe
rod
Actinomyces europaeus
28
0.10
+
facultative anaerobe
rod
Ferrimonas spp.
27
0.10
-
facultative anaerobe
rod
Bacillus spp.
24
0.09
+
aerobe
rod
UWB
22
0.08
Unk
unk
unk
Fusobacterium spp.
22
0.08
-
anaerobe
rod
Alcaligenes faecalis
20
0.07
-
aerobe
rod
UWB
17
0.06
Unk
unk
unk
Riemerella spp.
15
0.05
-
aerobe
rod
UWB
14
0.05
Unk
unk
unk
Stenotrophomonas spp.
14
0.05
-
aerobe
rod
Shewanella spp.
11
0.04
-
facultative anaerobe
rod
Eubacterium spp.
10
0.04
+
anaerobe
rod

This table provides the identified genus of the bacteria found using pyrosequencing (PRAPS) in the P sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. * The asterisk identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the P pool. Bacteria that were cultured and were not found using PRAPS include Acinetobacter, Leclercia, Morganella, and Pseudomonas. The % represents the percentage of the total sequences analyzed within the sample.

Dowd et al. BMC Microbiology 2008 8:43   doi:10.1186/1471-2180-8-43