Table 2

Results obtained for venous leg ulcer sample using pyrosequencing (PRAPS). This table provides the identified genera of the bacteria found using pyrosequencing (PRAPS) in the V sample, the number of sequences corresponding to a given genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the relative shape (rod or cocci) of the genus.

Genus

Seq

%

Gram

Aerotolerance

Shape


Enterobacter spp.

14288

44.83

-

Facultative anaerobe

Rod

Serratia spp. *

6132

19.24

-

Facultative anaerobe

Rod

Stenotrophomonas spp.

3532

11.08

-

Aerobe

Rod

Proteus spp.

2469

7.75

-

Facultative anaerobe

Rod

UWB

1446

4.54

unk

Unk

Unk

Proteus mirabilis.

1080

3.39

-

Facultative anaerobe

Rod

Salmonella spp.

739

2.32

-

Facultative anaerobe

Rod

Clostridium spp.

408

1.28

+

Anaerobe

Rod

Alcaligenes faecalis

337

1.06

-

Aerobe

Rod

UWB

261

0.82

unk

Unk

Unk

Pseudomonas spp.*

185

0.58

-

Aerobe

Rod

Staphylococcus spp.*

143

0.45

+

Facultative anaerobe

Cocci

Brevundimonas diminuta

123

0.39

-

Aerobe

Rod

Streptococcus spp.

107

0.34

+

Facultative anaerobe

Cocci

Acinetobacter spp.

102

0.32

-

Aerobe

Rod

Enterococcus spp.

94

0.29

+

Facultative anaerobe

Cocci

Pantoea spp.

81

0.25

-

Facultative anaerobe

Rod

Corynebacterium striatum*

81

0.25

+

Aerobe

Rod

Peptoniphilus spp.

65

0.20

+

Anaerobe

Cocci

E. coli

31

0.10

-

Facultative anaerobe

Rod

Bacillus spp.

25

0.08

+

Aerobe

Rod

Paenibacillus spp.

24

0.08

+

Facultative anaerobe

Rod

Eubacterium spp.

24

0.08

+

Anaerobe

Rod

Klebsiella spp.

21

0.07

-

Facultative anaerobe

Rod

Xanthomonas spp.

17

0.05

-

Aerobe

Rod

UWB

17

0.05

unk

Unk

Unk

Ferrimonas spp.

17

0.05

-

Facultative anaerobe

Rod

Finegoldia magna

13

0.04

+

Anaerobe

Cocci

Dendrosporobacter quercicolus

13

0.04

-

Anaerobe

Rod


UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. * The asterisk identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the V pool. The only bacterium cultured that was not found using PRAPS was Citrobacter. The % represents the percentage of the total sequences analyzed within the sample.

Dowd et al. BMC Microbiology 2008 8:43   doi:10.1186/1471-2180-8-43

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