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Open AccessHighly AccessResearch article

Rapid dissemination of Francisella tularensis and the effect of route of infection

Sandra S Ojeda1* email, Zheng J Wang2* email, Chris A Mares1 email, Tingtung A Chang3 email, Qun Li5 email, Elizabeth G Morris5 email, Paul A Jerabek4 email and Judy M Teale1,5 email

Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX, 78229, USA

MPI Research, 54943 North Main Street, Mattawan, MI, 49071, USA

Department of Radiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX, 78229, USA

Research Imaging Center, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX, 78229, USA

Department of Biology, University of Texas at San Antonio, One UTSA Circle San Antonio, TX, 78249, USA

author email corresponding author email* Contributed equally

BMC Microbiology 2008, 8:215doi:10.1186/1471-2180-8-215

Published: 9 December 2008

Additional files


Additional file 1:

Movie representing in vivo localization of64Cu labeledF. tularensis subsp. novicida following i.n infection. MicroPET and CT video of a mouse infected i.n with 64Cu labeled F. tularensis subsp. novicida 0.25 hrs p.i. The mouse shown in this movie corresponds to one different than the one depicted in Figure 1.

Format: MPG Size: 12.2MB Download file

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Additional file 2:

Movie representing in vivo localization of64Cu labeled F. tularensis subsp. novicida following i.p infection. MicroPET and CT video of a mouse infected i.p with 64Cu labeled F. tularensis subsp. novicida 0.25 hrs p.i. The mouse shown in this movie corresponds to one different than the one depicted in Figure 1.

Format: MPG Size: 8.8MB Download file

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Additional file 3:

Movie representing in vivo localization of64Cu labeled F. tularensis subsp. novicida following i.v infection. MicroPET and CT video of a mouse infected i.v with 64Cu labeled F. tularensis subsp. novicida 0.25 hrs p.i. The mouse shown in this movie corresponds to one different than the one depicted in Figure 1.

Format: MPG Size: 11.8MB Download file

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Additional file 4:

Movie representing in vivo localization of64Cu labeled F. tularensis subsp. novicida following i.t and i.g infections. MicroPET video of two mice, one infected i.t with 64Cu labeled F. tularensis subsp. novicida at 0.25 hrs p.i (left when the image is still) and another one infected i.g with 64Cu labeled F. tularensis subsp. novicida 0.25 hrs p.i (right when the image is still). The mice shown in this movie correspond to mice different than the ones depicted in Figure 1.

Format: MPG Size: 168KB Download file

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Additional file 5:

Movie representing in vivo localization of64Cu labeled F. tularensis subsp. novicida following i.d infection. MicroPET video of two mice infected i.d with 64Cu labeled F. tularensis subsp. novicida 0.25 hrs p.i (right and left). One of the mice shown in this movie corresponds to the one depicted in Figure 1 while the other mouse corresponds to another one imaged at the same time.

Format: MPEG Size: 169KB Download file

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Additional file 6:

Graph representing bacterial load following i.n infection with64Cu labeled F. tularensis subsp. novicida. In order to determine the presence of viable 64Cu labeled F. tularensis subsp. novicida in different tissues after infection, bacterial burden assays were performed. Data represent the mean with range of two mice and are given as Log CFU/g of tissue. Lung, liver, bladder and blood were harvested at 20 hrs p.i. 64Cu labeled F. tularensis subsp. novicida was isolated from all the tissues analyzed at this time point.

Format: TIFF Size: 427KB Download file


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