Table 2

B. mandrillaris RNase P genomic DNA in infected brain tissues detected by real-time PCR

DNA

[pg] ^c)

Amoebae

equivalents ^d)

CT ^e)

Murine brain tissue from non-infected mice

> 500

neg

≥ 39

Murine brain tissue from B.m.-infected mice ^a)

n.d.

n.d.

30

Human brain tissue from non-infected patients

n.d.

neg

≥ 39

Human brain tissue spiked with 50 B.m.

n.d.

50

29

Human brain tissue from BAE-patient 1 ^b) (1:100)

n.d.

n.d.

30

Human brain tissue from BAE-patient 2 ^b) (1:10)

n.d.

n.d.

35

^a) A highly symptomatic CD4⁺ T cell-deficient mouse 14 days post intranasal infection with B. mandrillaris amoebae. ^b) DNA extracted from 2 fresh BAE-patient brain biopsy samples ([23] Table 1: cases 4 and 5). ^c) Minimal amounts of total DNA giving positive results (CT < 39). ^d) Minimal numbers of amoebae (calculated as amoeba DNA equivalents per PCR tube) giving positive results; neg = CT ≥ 39 for at least 500 ng total DNA. ^e) Range of CT values measured; neg = CT ≥ 39 or no DNA detected; n.d. = not done.

Kiderlen et al. BMC Microbiology 2008 8:210   doi:10.1186/1471-2180-8-210