Figure 1.

Detection of B. mandrillaris RNase P (A)and 18S rRNA gene (B) DNA by duplex real-time PCR. Amplification curves of murine brain tissue (ca. 15 mm³) alone (closed diamonds) or spiked with 500 (closed squares), 50 (open squares), 5 (closed triangles), and 0,5 (open triangles) axenically cultured B. mandrillaris (calculated as amoeba DNA equivalents per PCR tube). Results from 3 separate experiments.