Table 2

Comparison between Y. pestis product sizes inferred by Agilent 2100 Bioanalyzer software (OBSERVED SIZE) and actual sizes obtained by direct sequencing of the PCR product or data available in Genbank (EXPECTED SIZE). UL BPS (unit length size).
MULTIPLEX
 MARKER (final primer concentration, μM)
 UL BPS
 ALLELE
 EXPECTED SIZE
 OBSERVED SIZE
 OFFSET
1
 ypms51 (0.2)
 21
 1
 186
 185
 1



2
 207
 207
 0

ypms01* (0.2)
 18
 6
 283
 279
 4



7
 301
 291
 10



9
 337
 329
 8



10
 355
 344
 11
2
 ypms04 (0.2)
 17
 5
 179
 187
 -8



6
 196
 205
 -9



8
 230
 241
 -11



9
 247
 259
 -12

ypms62* (0.2)
 9
 5
 270
 258
 12



6
 279
 267
 12



9
 306
 285
 21



10
 315
 294
 21



12
 333
 308
 25



15
 360
 331
 29



20
 405
 366
 39
3
 ypms07 (0.2)
 10
 7
 164
 168
 -4



8
 174
 178
 -4



9
 184
 187
 -3

ypms05 (0.2)
 17
 10
 274
 285
 -11



11
 291
 300
 -9
4
 ypms74 (0.2)
 15
 5
 180
 178
 2



6
 195
 190
 5



7
 210
 203
 7



8
 225
 217
 8

ypms15* (0.2)
 15
 9
 275
 272
 3



10
 290
 284
 6
5
 ypms35 (0.2)
 15
 8
 204
 208
 -4



9
 219
 222
 -3

ypms40* (0.2)
 17
 7
 253
 252
 1



8
 270
 266
 4



9
 287
 277
 10
6
 ypms38 (0.2)
 16
 6
 201
 200
 1



7
 217
 215
 2



8
 233
 230
 3

ypms20* (0.2)
 15
 8
 288
 285
 3



9
 303
 300
 3
7
 ypms41 (0.2)
 17
 5
 183
 188
 -5



6
 200
 207
 -7



7
 217
 221
 -4

ypms56* (0.2)
 16
 7
 258
 265
 -7



9
 290
 296
 -6
8
 ypms45 (0.2)
 12
 6
 149
 147
 2



7
 161
 159
 2

ypms44 (0.2)
 17
 7
 233
 239
 -6



8
 250
 260
 -10

ypms09* (0.2)
 18
 22
 732
 671
 61



32
 930
 847
 83



35
 984
 912
 72
9
 ypms71 (0.2)
 14
 5
 157
 163
 -6



6
 171
 178
 -7

ypms46 (0.2)
 7
 3
 238
 243
 -5



4
 245
 250
 -5



5
 252
 256
 -4



6
 259
 263
 -4



10
 308
 313
 -5



12
 322
 326
 -4
10
 ypms70 (0.2)
 9
 5
 137
 145
 -8



6
 146
 155
 -9



8
 164
 174
 -10



9
 173
 185
 -12



11
 191
 205
 -14

ypms54 (0.2)
 22
 6
 259
 265
 -6



7
 281
 289
 -8
11
 ypms69 (0.2)
 16
 5
 163
 164
 -1



6
 179
 177
 2



7
 195
 195
 0

ypms06 (0.2)
 60
 3
 306
 309
 -3



4
 366
 374
 -8



6
 486
 504
 -18



8
 606
 625
 -19



9
 666
 674
 -8
12
 ypms73 (0.2)
 18
 5
 207
 212
 -5



6
 225
 231
 -6

ypms21* (0.2)
 18
 9
 329
 339
 -10



10
 347
 358
 -11



11
 365
 375
 -10

* modified primer set (increased final fragment size)

Ciammaruconi et al. BMC Microbiology 2008 8:21   doi:10.1186/1471-2180-8-21