Table 1

Quantification of HtrA immunofluorescence (as a ratio of LPS) during penicillin and IFN-γ medicated persistence compared to untreated (acute) controls

Acute

Penicillin

Acute

IFN-γ


20 h PI

20 h PI

1.1a (0.08b)

0.84 (0.089)

0.74&(0.09)

0.62 (0.05)

44 h PI

44 h PI

1.49 (0.23)

1.85 (0.052)

1.06 (0.04)

0.33 (0.13)


a Each number represents the ratio of HtrA to LPS immunofluorescence in >10 individual inclusions dual labelled with LPS (FITC) and HtrA (561) antibodies. Quantification of total fluorescence was conducted under identical laser and microscopy settings on slides prepared on the same day (acute and persistence model).

b Standard error measurements are shown in brackets beside each Figure.

&Immunofluorescence for IFN-γ cultures was conducted with a higher laser power setting for laser 561 collections to enable accurate calculations of the HtrA measurements. Due to this difference control acute ratios are shown for both conditions.

Huston et al. BMC Microbiology 2008 8:190   doi:10.1186/1471-2180-8-190

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